Producción y bioactividades de los exopolisacáridos de Lactiplantibacillus plantarum BAL-29-ITTG utilizando un diseño experimental Plackett-Burman
DOI:
https://doi.org/10.31644/RMI.V3N3.2023.A03Palabras clave:
Antibiopelícula, Actividad Antioxidante, Bacterias Ácido Lácticas, Exopolisacáridos, Lactiplantibacillus plantarum BAL-29-ITTGResumen
Los polisacáridos microbianos son biopolímeros biodegradables y biocompatibles producidos por bacterias, hongos y levaduras. La producción de exopolisacáridos (EPS) por bacterias ácido lácticas (BAL) ha ganado un interés especial durante la última década debido a las propiedades funcionales de estos biopolímeros. El objetivo de este trabajo fue evaluar el efecto de las condiciones de cultivo sobre la producción de EPS provenientes de Lactiplantibacillus plantarum BAL-29-ITTG, así como su actividad antibiopelícula y antioxidante. Los factores estudiados fueron las fuentes de carbono y nitrógeno, sus concentraciones, la temperatura y la velocidad de agitación. Utilizando un diseño experimental Plackett Burman con 12 tratamientos, se determinó que la fuente y concentración de nitrógeno tuvieron un impacto estadísticamente significativo en la producción de EPS, alcanzando su máximo (619.66 mg/L) en un medio con lactosa a 10 g/L y extracto de levadura a 15 g/L, incubado a 20 °C sin agitación. La actividad antioxidante varió entre 63.2% y 98.64%, mientras que la actividad antibiopelícula sobre E. coli, S. aureus y P. aeruginosa fue influenciada significativamente por la fuente, concentración de nitrógeno y velocidad de agitación. Estas propiedades hacen que los EPS estudiados sean candidatos prometedores para aplicaciones en la industria farmacéutica, alimentaria y cosmética.
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